Sapstain fungi,which are a main cause for wood discoloration,reduce the value of logs,lumber and even the mechanical performance.Water immersion and chemical treatment are commonly-used methods to prevent sapstain fungi.A biocontrol method of using microbes to inhabit sapstain fungi was described in this paper.The microbes were developed from isolated sapstain fungi grown on birch wood logs（Betula platyphylla）.A total of 42 biocontrol microbes were identified having antagonistic effects on sapstain fungi to certain degrees.Among them,two were found to be very effective to prevent all sapstain fungi.The results would provide help and guidance for future studies.