In order to make environment-friendly cork floors with antimicrobia and antibacterial functions,two antibacterial agents（a nano silver compound and a titanium dioxide silver compound） were added into the water-based coating paint. The effects of the type and quantity of antibacterial agents on the floor performance were analyzed. The results showed that the both antibacterial agents had no effect on the floor physical properties, but they improved the antibacterial performance of cork floors significantly. A better anti-Staphylococcus aureus action was observed than the anti-Escherichia coli action. Cork flooring is naturally mold and mildew resistant, while the both added antibacterial agents showed a weak mildew resistant effect. It was suggested that the improvement of antimicrobia performance could be obtained by the addition of little mildew-resistant agent.