Effects of the modulus of elasticity（MOE）, layer numbers, thickness of timber and assembly directions on interlamination shear properties of cross-laminated timber（CLT） made from domestic Japanese larch in China were analyzed.The results showed that there was no significant effect on interlamination shear modulus but significant effect on interlamination shear strength of the CLT with increased MOE as well as decreased layer numbers and thickness of timber. The interlamination shear properties were significantly improved by changing timber assembles directions. The effective shear stiffness could be used to predict by the shear analogy method with less than 7% relative error between predicted and measured values.