降香黄檀DNA提取及其rDNA-ITS序列分子系统发育分析

李奇威; 谭贵良; 孙瑾; 苏越骁; 王业胜; 周林; 朱爽

doi:10.19455/j.mcgy.2016.03.006

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降香黄檀DNA提取及其rDNA-ITS序列分子系统发育分析
DNA Extractions from Dalbergia odorifera and a Molecular Phylogenetic Analyses Using Its rDNA-ITS Sequence
1. 广东药科大学生命科学与生物制药学院
2. 广东省生物技术候选药物研究重点实验室
3. 中山市质量计量监督检测所
4. 华南农业大学林学院
2016年30卷第3期页码：21-24
DOI： 10.19455/j.mcgy.2016.03.006

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[1]李奇威,谭贵良,孙瑾,苏越骁,王业胜,周林,朱爽.降香黄檀DNA提取及其rDNA-ITS序列分子系统发育分析[J].木材工业,2016,30(03):21-24.

LI Qi-wei, TAN Gui-liang, SUN Jin, et al. DNA Extractions from Dalbergia odorifera and a Molecular Phylogenetic Analyses Using Its rDNA-ITS Sequence[J]. China Wood Industry, 2016,30(3):21-24.
[1]李奇威,谭贵良,孙瑾,苏越骁,王业胜,周林,朱爽.降香黄檀DNA提取及其rDNA-ITS序列分子系统发育分析[J].木材工业,2016,30(03):21-24. DOI： 10.19455/j.mcgy.2016.03.006.

LI Qi-wei, TAN Gui-liang, SUN Jin, et al. DNA Extractions from Dalbergia odorifera and a Molecular Phylogenetic Analyses Using Its rDNA-ITS Sequence[J]. China Wood Industry, 2016,30(3):21-24. DOI： 10.19455/j.mcgy.2016.03.006.

摘要

以核糖体转录间隔区（rDNA-ITS）序列为分子标记,结合GenBank数据库中已有序列,阐明黄檀属内不同物种之间的分子系统发育关系。通过改良十六烷基三甲基溴化铵法（CTAB）提取降香黄檀总DNA,利用引物对rDNA-ITS序列进行聚合酶链式反应（PCR扩增）和序列测定。结果表明,基于rDNA-ITS序列测定、位点分析和系统发育树构建方法,可利用ITS序列进行黄檀属树种准确的分子鉴定,为其种类鉴定和种间分类地位提供分子生物学依据。

Abstract

To clarify phylogenetic relationships among different species of genus Dalbergia, genetic analysis was carried out for Dalbergia odorifera using its r DNA-ITS sequence as molecular markers. Total DNA was extracted with a modified CTAB method, and r DNA-ITS regions were amplified with universally conserved primers. The results showed that, based on molecular biology methods of r DNA-ITS sequence analysis, accurate molecular identification could be realized in genus Dalbergia so as to provide molecular evidence for taxonomy and identification of different species in genus Dalbergia.

关键词

降香黄檀DNA提取ITS序列分子鉴定

Keywords

Dalbergia odoriferaDNA extractionITS sequencemolecular identification

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