Domestic Japanese larch lumber was used to prepare cross-laminated timber（CLT） samples. Modulus of elasticity（MOE） was tested using a static method and longitudinal vibration. Shear analogy was also used to predict effective bending stiffness. Feasibility and accuracy of nondestructive testing technology and theoretical calculation were analyzed.Testing results showed that:1)MOE was improved by increasing the span/depth ratio and should be more than 24;2)Dynamic MOE was higher than static MOE with less than 10%of relative error;3)Effective bending stiffness of the CLT samples was predicted by shear analogy with less than 5%of relative error between predicted and measured values.