The authors developed a facile one step sol-gel method with TEOS as a precursor and KH550 as a modifier for preparation of a superhydrophobic surface on wood substrate which aims to simplify previous preparation methods. Surface morphology and water repellency of the treated sample were tested, respectively. The results indicated that the micro-nano hierarchical structures, similar to lotus leaves, combining with low-surface-free-energy material transformed the treated Chinese fir samples from a hydrophilic surface to a superhydrophobic surface, with a contact angle(CA) of 151.5°and a sliding angle lower than 8°. After exposure in an air environment for one year, then being immersed in acidic and alkali solutions for 14 hours, washed by an ultrasonic environment for 25 min, the sample’s surface still possessed great hydrophobic property with CA larger than 140°.