高效液相色谱法测定防腐材中嘧菌酯含量的方法研究

张景朋; 邵闯; 蒋明亮

doi:10.12326/j.2096-9694.2024076

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高效液相色谱法测定防腐材中嘧菌酯含量的方法研究
Measurement of the Azoxystrobin Content in Preservative-Treated Wood by High Performance Liquid Chromatography
中国林业科学研究院木材工业研究所，北京 100091
2025年39卷第1期页码：64-70
DOI： 10.12326/j.2096-9694.2024076
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张景朋,邵闯,蒋明亮.高效液相色谱法测定防腐材中嘧菌酯含量的方法研究[J].木材科学与技术,2025,39(01):64-70. DOI： 10.12326/j.2096-9694.2024076.

ZHANG Jingpeng,SHAO Chuang,JIANG Mingliang.Measurement of the Azoxystrobin Content in Preservative-Treated Wood by High Performance Liquid Chromatography[J].Chinese Journal of Wood Science and Technology,2025,39(01):64-70. DOI： 10.12326/j.2096-9694.2024076.

摘要

为实现防腐处理材中嘧菌酯（azoxystrobin）含量的准确快捷检测，首先建立嘧菌酯的高效液相色谱（HPLC）分析方法（紫外检测器及C18反相色谱柱，

（甲醇）∶

（水）=3

∶1为流动相，流速0.8 mL/min，检测波长220 nm），然后利用超声波提取木/竹材中的防腐剂，并优化提取的条件。结果表明：嘧菌酯在质量浓度10 ~ 120 mg/L的范围内，标准曲线的决定系数为0.999 7，线性相关性显著，分析样品的变异系数为1.39%和1.55%，样品的回收率为96.5% ~ 101.6%。在优化的超声波提取条件下：溶剂配比为

（甲醇）∶

（水）=8∶2，提取时间60 min，2个载药量水平，辐射松（

Pinus radiata

）、马尾松（

Pinus massoniana

）、杉木（

Cunninghamia lanceolata

）、和107杨（

Populus

euramericana

cv.‘74/76’）防腐木中嘧菌酯的回收率为95.5%~99.6%，标准偏差在0.8% ~ 3.8%范围内；毛竹（

Phyllostachys edulis

）提取回收率为84.4%和88.3%。本方法可用于检测处理材中嘧菌酯的含量，前处理步骤简便快捷，分析结果准确度高，重现性好。

Abstract

In order to measure the content of azoxystrobin in treated wood precisely

a high performance liquid chromatography (HPLC) analysis method was constructed using a UV detector and C18 reversed-phase chromatography column

the

(methanol)∶

(water)=3∶1 as the mobile phase

with a flow rate of 0.8 mL/min and UV detection wavelength of 220 nm. Then

the ultrasonic extraction was used for pre-treatment of preservative extraction in wood/bamboo

and the extraction conditions were optimized. The results showed that the determination coefficient of the standard curve for azoxystrobin was 0.999 7 within the mass concentration range of 10~120 mg/L

and the linear correlation was significant. The coefficients of variation were 1.39% and 1.55%

and the recovery rates were 96.5%~101.6%. Under the optimized ultrasonic extraction conditions

including the solvent ratio of

(methanol)∶

(water)=8∶2

the extraction time of 60 minutes

and two retention levels

the azoxystrobin recovery rate of Radiata pine

masson pine

Chinese fir

and poplar‘107’was 95.5%~99.6%

with a standard deviation of 0.8%~3.8%. The recovery rates of Moso bamboo extraction were 84.4% and 88.3%

respectively. This method can be used to measure the azoxystrobin content in treated materials

with simple and fast pretreatment steps

achieving a hi

gh precision of analysis results and the good reproducibility.

关键词

Keywords

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醚菌酯高效液相色谱分析方法及其防腐材抗流失性能研究