Analysis of Microbial Community Structure in the Wood Piles from the Yalu River Floating Bridge Site

HAN Xu; LI Hui; WANG Yuchen; ZHOU Haibin; DENG Hao

doi:10.12326/j.2096-9694.2024137

KEY SCIENTIFIC RESEARCH BASE OF HISTORICAL BUILDING WOOD | Views : 0 下载量: CSCD: 0 CNKI被引量: 0 Crossref: 0 Wos: 0 Scopus: 0 更多指标

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Analysis of Microbial Community Structure in the Wood Piles from the Yalu River Floating Bridge Site
1.Research Institute of Wood Industry，Chinese Academy of Forestry，Beijing 100091，China
2.Dandong Cultural Tourism and Radio and Television Bureau，Dandong 118000，Liaoning，China
Vol. 39, Issue 2, Pages: 1-11(2025)
DOI： 10.12326/j.2096-9694.2024137
稿件说明：

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韩旭,李辉,王雨晨等.鸭绿江浮桥遗址木桩表层微生物群落结构分析[J].木材科学与技术,2025,39(02):1-11. DOI： 10.12326/j.2096-9694.2024137.

HAN Xu,LI Hui,WANG Yuchen,et al.Analysis of Microbial Community Structure in the Wood Piles from the Yalu River Floating Bridge Site[J].Chinese Journal of Wood Science and Technology,2025,39(02):1-11. DOI： 10.12326/j.2096-9694.2024137.

摘要

鸭绿江浮桥遗址是我国革命历史的重要见证，遗址作为在江面保存的木质不可移动文物，系统分析微生物群落是科学保护文物的基础。研究选择江中桥墩木桩不浸水区域I、感潮区域Ⅱ、长期浸水区域Ⅲ等三个区域，观察各区域不同部位样品的劣化情况，并采集表层微生物样品，通过Illumina MiSeq 高通量测序技术进行微生物群落分析。结果显示，木桩不同部位产生了不同程度的材质老化、微生物降解现象，不浸水区域I木材存在老化变色、材质疏松、表层剥落等严重劣化现象，其主要成分降解严重；感潮区域Ⅱ中也可见木材老化变色、剥落现象，但劣化程度较轻；长期浸水区域Ⅲ中木材保存状况较好。木桩细菌群落在门、目、属水平上丰度较高的主要为变形菌门（Proteobacteria）、鞘脂单胞菌目（Sphingomonadales）、鞘氨醇单胞菌属（

Sphingomonas

），真菌群落在门、目、属水平上丰度较高的主要为子囊菌门（Ascomycota）、伞菌目（Agaricales）、鬼伞属（

Coprinus

）。木桩不浸水区域I中主要真菌为伞菌目真菌，而长期浸水区域Ⅲ主要真菌为刺盾炱目（Chaetothyriales）真菌，木桩不同区域主要细菌均为鞘脂单胞菌目细菌与根瘤菌目（Rhizobiales）细菌。研究结果对鸭绿江浮桥遗址桥墩木桩预防性和适宜性保护提供科学依据。

Abstract

The Yalu River Floating Bridge Site is an important witness of China’s revolutionary history. As an unmov

able wooden cultural relic preserved at the river surface

the systematic analysis of microbial communities provides scientific foundation for protecting the cultural relics. In this study

three parts from the non-immersed area I

the tidal area Ⅱ

and the long-term immersed area Ⅲ of the wooden pile in the middle of the river were selected. It was observed of the microbial erosion in the wood structure of the selected samples

which are the immersed part of the wooden pile in the middle of the river. The Illumina MiSeq high-throughput sequencing platform was used for sequencing and analysis. The results show that samples from different locations of the wood pile present various degrees of natural aging and microbial degradation. There are serious degradation phenomena such as aging discoloration

loose material

and surface peeling in the non-immersed area. The main components degraded most seriously. The phenomenon of aging discoloration and wood peeling is also observed in the samples from the tidal area

while the degree of deterioration is less. The samples from the long-term immersion area Ⅲ are well preserved. Proteobacteria

Sphingomonadales

and

Sphingomonas

are the main bacteria species with high abundance at the levels of phylum

order

and genus. Ascomycota

Agaricales

and

Coprinus

were the main fungus species with high abundance at the levels of phylum

order

and genus. The main fungi found in the non-immersed area I of the pile are Agaricales

while the main fungi found in the long-term submerged area Ⅲ are Scutellaceae. The main bacteria identified at different locations of the pile were Sphingomonas and Rhizobiales. The research results provide scientific basis for the preventive and suitability protection of wooden piles at the Yalu River floating bridge site.

关键词

Keywords

references

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